anti cd4 pe (Miltenyi Biotec)
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Anti Cd4 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd4+pe/CD4+Antibody%2C+anti-mouse%2C+REAfinity/pmc13355016-7-0-2
Average 95 stars, based on 123 article reviews
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Bioprocessing:Article Title: Ex vivo enhancement of CD8+ T cell activity using functionalized hydrogel encapsulating tonsil-derived lymphatic endothelial cells Article Snippet: .. The following anti-human monoclonal antibodies were used in this study: 1) CD44 PE, CD73 APC, CD90 FITC, CD105PerCP-Cy5.5, and CD11b/CD19/CD34/CD45/HLA-DR PE (Human MSC Analysis Kit #562245, BD Biosciences) for TMSCs; 2) podoplanin APC, VEGF-R3 PE (both Biolegend), ALEXA FLUOR ® 488-conjugated Prox-1 (Bioss, Woburn, MA., USA), and ALEXA FLUOR ® 488-conjugated LYVE-1 (R&D Systems, Minneapolis, MN., USA) for T-LECs; 3) Article Title: Longitudinal Assessment of Multiple Immunological and Inflammatory Parameters during Successful DAA Therapy in HCV Monoinfected and HIV/HCV Coinfected Subjects Article Snippet: Lysing solution (BD Biosciences Pharmagen, Milano, Italy) was added to the samples, and after additional 10 min of incubation, the samples were analyzed using a MACS Quant flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Cologne, Germany). .. The anti-human monoclonal antibodies used were CD45-VioBlue, HLA-DR-FITC, CD38-APC, CD8-PerCP and Article Title: Targeting the melanoma-associated antigen CSPG4 with HLA-C*07:01-restricted T-cell receptors. Article Snippet: .. The following monoclonal antibodies were used: Staining:Article Title: From DGCR8 expression analysis to diseased pathways in 22q11.2 deletion syndrome Article Snippet: Thawed PBMCs of patients and healthy controls, obtained as described above, were treated with IFN2alpha (300 U/mL, Miltenyi Biotech) for 48 and 72 hours at 37°C, 5% CO2. .. Cells were surface stained with CD3 VioBlue (Miltenyi), Article Title: Therapeutic activity of retroviral replicating vector-mediated gene therapy in combination with anti-PD-1 antibody in a murine pancreatic cancer model. Article Snippet: Toca 511, a tumor-selective retroviral replicating vector encoding the yeast cytosine deaminase (yCD) gene, exerts direct antitumor effects through intratumoral prodrug 5-fluorocytosine (5-FC) conversion to active drug 5-fluorouracil by yCD, and has demonstrated therapeutic efficacy in preclinical and clinical trials of various cancers.. Toca 511/5-FC treatment may also induce antitumor immunity.. Here, we first examined antitumor immune responses activated by Toca 511/5-FC treatment in an immunocompetent murine pancreatic cancer model. We then evaluated the therapeutic effects achieved in combination with anti-programmed cell death protein 1 antibody. Flow Cytometry:Article Title: Therapeutic activity of retroviral replicating vector-mediated gene therapy in combination with anti-PD-1 antibody in a murine pancreatic cancer model. Article Snippet: Toca 511, a tumor-selective retroviral replicating vector encoding the yeast cytosine deaminase (yCD) gene, exerts direct antitumor effects through intratumoral prodrug 5-fluorocytosine (5-FC) conversion to active drug 5-fluorouracil by yCD, and has demonstrated therapeutic efficacy in preclinical and clinical trials of various cancers.. Toca 511/5-FC treatment may also induce antitumor immunity.. Here, we first examined antitumor immune responses activated by Toca 511/5-FC treatment in an immunocompetent murine pancreatic cancer model. We then evaluated the therapeutic effects achieved in combination with anti-programmed cell death protein 1 antibody. Article Title: Monocyte infiltration induces CNS arginine catabolism to fuel neuroinflammation. Article Snippet: .. Flow cytometry and cell sorting For analysis of spinal cord cell populations, stainings were performed in PBS using fixable viability dye (Invitrogen, 65-0865-14, 1:2,000, 10 min at room temperature) as well as the following antibodies: CD45.2-BV650 (clone 104, Biolegend, 109836, 1:100), Ly6G-BV605 (clone 1A8, Biolegend, 127639, 1:100), Ly6G-AF700 (clone 1A8, Biolegend, 127622, 1:100), CD3-BV605 (clone 145-2C11, Biolegend, 100351, 1:100), Ly6C-BV605 (clone HK14, Biolegend, 128035, 1:100), Ly6C-BV510 (clone HK14, Biolegend, 128033, 1:100), CD11b-BV510 (clone M1/70, Biolegend, 101245, 1:100), CD11b-PB (clone M1/70.15, Invitrogen, RM2828, 1:100), CD11b-BV605 (clone M1/70, Biolegend, 101237, 1:100), CX3CR1-PeCy7 (clone SA011F11, Biolegend, 149015, 1:100), CCR2-BV421 (clone SA203G11, Biolegend, 150605, 1:100), CCR2-FITC (clone SA203G11, Biolegend, 150607, 1:100), F4/80-BV510 (clone BM8, Biolegend, 123135, 1:100), F4/80-BV605 (clone BM8, Biolegend, 123133, 1:100), F4/80-FITC (clone BM8, Biolegend, 123108, 1:100), CD11c-PE-Cy5.5 (clone N418, Invitrogen, 35-0114-82, 1:100), P2RY12-APC (clone S16007D, Biolegend, 848006, 1:100), P2RY12-AF488 (clone S16007D, Biolegend, 848016, 1:100), MerTK-APC (clone 2B10C42, Biolegend, 151507, 1:100), CD44-FITC (clone IM7, Invitrogen, 11-0441-82, 1:100), CD44-eF506 (clone IM7, Invitrogen, 69-0441-82, 1:100), CD64-BV605 (clone X545/7.1, Biolegend, 139323, 1:100), CD3-AF700 (clone 17A2, Biolegend, 100216, 1:100), CD3-rF710 (clone 17A2, Tonbo, 80-0032-U100, 1:80), CD8-BV510 (clone 53-6.7, Biolegend, 100751, 1:80), CD4-APC (clone REA604, Miltenyi Biotec, 130-116-487, 1:80), Article Title: Patterns of Differentially Expressed circRNAs in Human Thymocytes Article Snippet: .. All the immunophenotypes of the thymocyte populations were magnetically sorted and validated by flow cytometry using a FACSCalibur cytometer (Becton Dickinson, San Diego, CA, USA) with the following antibodies: CD34-PE, CD8-APC and Control:Article Title: Therapeutic activity of retroviral replicating vector-mediated gene therapy in combination with anti-PD-1 antibody in a murine pancreatic cancer model. Article Snippet: Toca 511, a tumor-selective retroviral replicating vector encoding the yeast cytosine deaminase (yCD) gene, exerts direct antitumor effects through intratumoral prodrug 5-fluorocytosine (5-FC) conversion to active drug 5-fluorouracil by yCD, and has demonstrated therapeutic efficacy in preclinical and clinical trials of various cancers.. Toca 511/5-FC treatment may also induce antitumor immunity.. Here, we first examined antitumor immune responses activated by Toca 511/5-FC treatment in an immunocompetent murine pancreatic cancer model. We then evaluated the therapeutic effects achieved in combination with anti-programmed cell death protein 1 antibody. FACS:Article Title: Monocyte infiltration induces CNS arginine catabolism to fuel neuroinflammation. Article Snippet: .. Flow cytometry and cell sorting For analysis of spinal cord cell populations, stainings were performed in PBS using fixable viability dye (Invitrogen, 65-0865-14, 1:2,000, 10 min at room temperature) as well as the following antibodies: CD45.2-BV650 (clone 104, Biolegend, 109836, 1:100), Ly6G-BV605 (clone 1A8, Biolegend, 127639, 1:100), Ly6G-AF700 (clone 1A8, Biolegend, 127622, 1:100), CD3-BV605 (clone 145-2C11, Biolegend, 100351, 1:100), Ly6C-BV605 (clone HK14, Biolegend, 128035, 1:100), Ly6C-BV510 (clone HK14, Biolegend, 128033, 1:100), CD11b-BV510 (clone M1/70, Biolegend, 101245, 1:100), CD11b-PB (clone M1/70.15, Invitrogen, RM2828, 1:100), CD11b-BV605 (clone M1/70, Biolegend, 101237, 1:100), CX3CR1-PeCy7 (clone SA011F11, Biolegend, 149015, 1:100), CCR2-BV421 (clone SA203G11, Biolegend, 150605, 1:100), CCR2-FITC (clone SA203G11, Biolegend, 150607, 1:100), F4/80-BV510 (clone BM8, Biolegend, 123135, 1:100), F4/80-BV605 (clone BM8, Biolegend, 123133, 1:100), F4/80-FITC (clone BM8, Biolegend, 123108, 1:100), CD11c-PE-Cy5.5 (clone N418, Invitrogen, 35-0114-82, 1:100), P2RY12-APC (clone S16007D, Biolegend, 848006, 1:100), P2RY12-AF488 (clone S16007D, Biolegend, 848016, 1:100), MerTK-APC (clone 2B10C42, Biolegend, 151507, 1:100), CD44-FITC (clone IM7, Invitrogen, 11-0441-82, 1:100), CD44-eF506 (clone IM7, Invitrogen, 69-0441-82, 1:100), CD64-BV605 (clone X545/7.1, Biolegend, 139323, 1:100), CD3-AF700 (clone 17A2, Biolegend, 100216, 1:100), CD3-rF710 (clone 17A2, Tonbo, 80-0032-U100, 1:80), CD8-BV510 (clone 53-6.7, Biolegend, 100751, 1:80), CD4-APC (clone REA604, Miltenyi Biotec, 130-116-487, 1:80), Cytometry:Article Title: Patterns of Differentially Expressed circRNAs in Human Thymocytes Article Snippet: .. All the immunophenotypes of the thymocyte populations were magnetically sorted and validated by flow cytometry using a FACSCalibur cytometer (Becton Dickinson, San Diego, CA, USA) with the following antibodies: CD34-PE, CD8-APC and Magnetic Cell Separation:Article Title: Patterns of Differentially Expressed circRNAs in Human Thymocytes Article Snippet: .. All the immunophenotypes of the thymocyte populations were magnetically sorted and validated by flow cytometry using a FACSCalibur cytometer (Becton Dickinson, San Diego, CA, USA) with the following antibodies: CD34-PE, CD8-APC and Purification:Article Title: Patterns of Differentially Expressed circRNAs in Human Thymocytes Article Snippet: .. All the immunophenotypes of the thymocyte populations were magnetically sorted and validated by flow cytometry using a FACSCalibur cytometer (Becton Dickinson, San Diego, CA, USA) with the following antibodies: CD34-PE, CD8-APC and |

